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Details

Instruments details

Genetic analyzer (Sanger sequencing) (Thermo) (External Samples)

Biochemical Characterization Facility

Equipment Details : The 3500xl genetic analyzer is a fluorescence-based utilizing capillary electrophoresis for DNA sequencing and fragment analysis. The 3500xl system uses a 24-capillary array and single-line 505 nm, solid-state long-life laser for significantly improved signal uniformity. Integrated software for instrument control, data collection, quality control, and auto-analysis of sample files for base calling and fragment sizing. Average throughput using POP7 and 50 cm array is 264 samples per day for sequ

Capabilities : Specifically designed to support sanger sequencing.

2417/2456
ncim@ncl.res.in
Installed on 2013

Scientist Incharge

Mahesh Dharne (2470)

Moderators

Ratnamala Valvi (2464)

Shalaka Gaikaiwari (2376)

Address

National Collection of Industrial Micro-Organisms Basement 42 Main Building

Requisition Form : SANGER SEQUENCING

Book Test

Analysis :

Id	Title	External Charges
101	Sequencing of non-purified PCR product(~600 bases)	2497
102	Sequencing of pure culture by 16S/18S/other (~1200 bases)	4400
103	Sequencing of pure culture by 16S/ITS/18S/D1-D2 (~600 bases)	3200
300	Sequencing of non-purified PCR product(~1200 bases)	3800
301	Sequencing of purified PCR product/cloned plasmid DNA (~600 bases)	1400
302	Sequencing of purified PCR product/cloned plasmid DNA (~1200 bases)	2500
303	Cycle sequenced ready to run samples 600 bases (only for 1000 samples and above)	300